1D7E
CRYSTAL STRUCTURE OF THE P65 CRYSTAL FORM OF PHOTOACTIVE YELLOW PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 40.561, 40.561, 118.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.390 |
| R-factor | 0.138 * |
| Rwork | 0.139 |
| R-free | 0.19800 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.920 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.440 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.039 | 0.126 |
| Total number of observations | 194171 * | |
| Number of reflections | 21796 * | 2190 * |
| <I/σ(I)> | 23.1 | |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 290 | used as seeds, Borgstahl, G.O., (1995) Biochemistry, 34, 6278. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 3.2 (M) | ||
| 2 | 1 | reservoir | sodium phosphate | 20 (mM) | |
| 3 | 1 | drop | protein | 15 (mg/ml) | |
| 4 | 1 | drop | 2.5 (M) | ||
| 5 | 1 | drop | sodium phosphate | 20 (mM) |
