1D64
CRYSTAL STRUCTURE OF A PENTAMIDINE-OLIGONUCLEOTIDE COMPLEX: IMPLICATIONS FOR DNA-BINDING PROPERTIES
Experimental procedure
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 292 |
Detector technology | AREA DETECTOR |
Detector | XENTRONICS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.370, 40.000, 66.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.100 |
R-factor | 0.194 |
RMSD bond length | 0.032 |
RMSD bond angle | 0.041 |
Refinement software | NUCLSQ |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.100 | 2.100 * |
Rmerge | 0.070 | |
Total number of observations | 10630 * | |
Number of reflections | 3824 | 353 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 289.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | NA CACODYLATE | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 10 (mg/ml) | |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | pentamidine | 25 (mM) | |
4 | 1 | drop | MPD | 50 (%(v/v)) | |
5 | 1 | drop | sodium cacodylate | 30 (mM) | |
6 | 1 | reservoir | 50 (%(v/v)) |