1D54
ANTHRACYCLINE BINDING TO DNA: HIGH RESOLUTION STRUCTURE OF D(TGTACA) COMPLEXED WITH 4'-EPIADRIAMYCIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 297 |
Detector technology | DIFFRACTOMETER |
Detector | RIGAKU AFC-5 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 28.020, 28.020, 52.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 1.400 |
R-factor | 0.17 |
RMSD bond length | 0.020 |
RMSD bond angle | 0.036 |
Refinement software | NUCLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.300 * |
Rmerge | 0.064 * |
Total number of observations | 11022 * |
Number of reflections | 5644 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 4 * | pH 6.50, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | MG ACETATE | ||
5 | 1 | 1 | SPERMINE_HCL | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 1 (mM) | |
2 | 1 | drop | 4'-epiadriamycin | 1.2 (mM) | |
3 | 1 | drop | sodium cacodylate | 50 (mM) | |
4 | 1 | drop | magnesium acetate | 140 (mM) | |
5 | 1 | drop | spermine hydrochloride | 0.6 (mM) | |
6 | 1 | drop | MPD | 18 (%) | |
7 | 1 | reservoir | 80 (%) |