1CWQ
M INTERMEDIATE STRUCTURE OF THE WILD TYPE BACTERIORHODOPSIN IN COMBINATION WITH THE GROUND STATE STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.080, 61.080, 110.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.000 - 2.250 |
| R-factor | 0.179 * |
| Rwork | 0.167 |
| R-free | 0.20800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.082 * | |
| Number of reflections | 10792 | |
| <I/σ(I)> | 12.05 | 3.4 |
| Completeness [%] | 97.2 | 99.4 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | cubic lipid phase * | 5.6 | 20 * | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium potassium Pi | 3.3 (M) | |
| 2 | 1 | 1 | protein | 3.5 (mg/ml) | |
| 3 | 1 | 1 | methylpentandiol | 0.05 (%) | |
| 4 | 1 | 1 | beta-octylglycopyranoside | 1.2 (%) |
