1CQ8
ASPARTATE AMINOTRANSFERASE (E.C. 2.6.1.1) COMPLEXED WITH C6-PYRIDOXAL-5P-PHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 157.720, 85.570, 78.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.213 * |
| Rwork | 0.213 |
| R-free | 0.27000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.091 | 0.291 |
| Total number of observations | 66489 * | |
| Number of reflections | 20719 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 97.4 | 94.2 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | ammonium sulfate, potassium phosphate, C6-pyridoxal-5p-phosphate,sodium azide, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 10 (mM) | |
| 3 | 1 | drop | PLP-amino acid | 0.010 (mM) | |
| 4 | 1 | drop | 0.3 (mM) | pH8.0 | |
| 5 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
| 6 | 1 | reservoir | potassium phosphate | 10 (mM) |
