1CPO
CHLOROPEROXIDASE
Experimental procedure
Collection date | 1994-04-28 |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.774, 71.363, 91.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.22400 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.557 |
Data reduction software | XENGEN |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.900 * |
Rmerge | 0.082 * |
Total number of observations | 152222 * |
Number of reflections | 29366 |
Completeness [%] | 94.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | Sundaramoorthy, M., (1995) Acta Crystallogr.,Sect.D, 51, 842. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 20 (%) | |
2 | 1 | reservoir | 0.31 (M) | can be replaced by LiCl or KCl | |
3 | 1 | reservoir | potassium phosphate | 0.01 (M) | |
4 | 1 | drop | PEG6000 | 10 (%) | |
5 | 1 | drop | 0.155 (M) | can be replaced by LiCl or KCl | |
6 | 1 | drop | potassium phosphate | 0.005 (M) | |
7 | 1 | drop | protein | 5 (mg/ml) | |
8 | 1 | drop | phosphate | 0.005 (M) |