1CGI

THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	84.400, 84.400, 86.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.195
RMSD bond length	0.018
RMSD bond angle	0.056
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.062 *
Total number of observations	27079 *
Number of reflections	10379 *
Completeness [%]	71.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme
2	1	drop	inhibitor	10 (mg/ml)
3	1	drop	Tris-HCl	50 (mM)
4	1	reservoir	ammonium sulfate	1.4-1.6 (M)