1CBS
CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.650, 47.560, 77.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.800 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.23700 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.510 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 * | |
High resolution limit [Å] | 1.800 * | 1.800 * |
Rmerge | 0.059 * | |
Total number of observations | 83399 * | |
Number of reflections | 14678 | |
Completeness [%] | 90.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein-ligand complex | 0.003ml | |
2 | 1 | drop | Tris | 20 (mM) | |
3 | 1 | drop | monomethyl polyethylene glycol 5000 | 10 (%(v/v)) | |
4 | 1 | drop | sodium citrate | 0.1 (M) | |
5 | 1 | drop | dimethylsulphoxide | 5 (%) | |
6 | 1 | reservoir | monomethyl polyethylene glycol 5000 | 10 (%(v/v)) | |
7 | 1 | reservoir | sodium citrate | 0.1 (M) | |
8 | 1 | reservoir | dimethylsulphoxide | 5 (%) |