1C9Y
HUMAN ORNITHINE TRANSCARBAMYLASE: CRYSTALLOGRAPHIC INSIGHTS INTO SUBSTRATE RECOGNITION AND CATALYTIC MECHANISM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-29 |
Spacegroup name | P 21 3 |
Unit cell lengths | 125.410, 125.410, 125.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.186 * |
Rwork | 0.187 |
R-free | 0.20600 * |
RMSD bond length | 0.012 |
RMSD bond angle | 23.960 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.149 |
Total number of observations | 799411 * | |
Number of reflections | 52412 | |
<I/σ(I)> | 54.3 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 16 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 18 * | PEG 400, AMMONIUM SULFATE, POTASSIUM CHLORIDE, EDTA, TRIS ACETATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | PALO | 4 (mM) | |
3 | 1 | drop | Tris acetate | 20 (mM) | |
4 | 1 | drop | EDTA | 2 (mM) | |
5 | 1 | drop | 20 (mM) | ||
6 | 1 | reservoir | PEG400 | 2 (%) | |
7 | 1 | reservoir | ammonium sulfate | 2 (M) | |
8 | 1 | reservoir | sodium HEPES | 0.1 (M) |