1C9P
COMPLEX OF BDELLASTASIN WITH PORCINE TRYPSIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.330, 63.330, 130.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.185 * |
| Rwork | 0.185 |
| R-free | 0.22700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.040 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.096 | 0.280 |
| Total number of observations | 31627 * | |
| Number of reflections | 7063 * | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.9 * | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | 21 * | 0.1M HEPES, 10% 2-PROPANOL, 20% PEG 4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | MES | 20 (mM) | |
| 4 | 1 | reservoir | 2-propanol | 10 (%(v/v)) | |
| 5 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 6 | 1 | reservoir | HEPPS | 100 (mM) |
