1C2A
CRYSTAL STRUCTURE OF BARLEY BBI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1994-06-20 |
Detector | FUJI |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.480, 62.480, 94.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.21900 |
RMSD bond length | 0.008 |
RMSD bond angle | 27.700 * |
Data reduction software | ROTAVATA |
Data scaling software | WEIS |
Phasing software | SHARP |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.058 | 0.194 |
Total number of observations | 133929 * | |
Number of reflections | 13611 | |
<I/σ(I)> | 15 | |
Completeness [%] | 88.0 | 74.1 |
Redundancy | 9.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.23 | 296 | Song, H.K., (1997) Acta Crystallogr., Sect., D54, 441. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1.0 (mg/ml) | |
2 | 1 | drop | PEG3000 | 23 (%(w/v)) | |
3 | 1 | drop | sodium citrate | 100 (mM) | |
4 | 1 | reservoir | PEG3000 | 36.5 (%(w/v)) | |
5 | 1 | reservoir | sodium citrate | 550 (mM) |