1BT6
P11 (S100A10), LIGAND OF ANNEXIN II IN COMPLEX WITH ANNEXIN II N-TERMINUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 280 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-25 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.600, 56.400, 64.300 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.233 |
| Rwork | 0.228 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4p |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.610 * |
| Total number of observations | 29481 * | |
| Number of reflections | 9774 | |
| <I/σ(I)> | 20.6 | 1.96 |
| Completeness [%] | 96.8 | |
| Redundancy | 6.25 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 3 | 1 | 1 | dithiothreitol | 1 (mM) | |
| 4 | 1 | 2 | PEG4000 | 10 (%) | |
| 5 | 1 | 2 | 2-propanol | 10 (%) | |
| 6 | 1 | 2 | Tris-HCl | 100 (mM) |
