1BSC

CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE

Experimental procedure

Spacegroup name	P 32
Unit cell lengths	58.710, 58.710, 81.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 * - 2.000
R-factor	0.166 *
RMSD bond length	0.017 *
RMSD bond angle	2.500 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.069 *
Completeness [%]	91.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	23 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	45 (%sat)
2	1	reservoir	ammonium phosphate	3.0 (M)
3	1	reservoir	zinc sulphate	0.02 (M)
4	1	drop	protein	10-20 (mg/ml)