1BR2
SMOOTH MUSCLE MYOSIN MOTOR DOMAIN COMPLEXED WITH MGADP.ALF4
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-01 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 134.010, 107.670, 188.310 |
Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.900 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mnd |
RMSD bond length | 0.009 |
RMSD bond angle | 25.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.120 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.072 * | 0.215 * |
Total number of observations | 326933 * | |
Number of reflections | 99071 | |
<I/σ(I)> | 28 | 14.5 |
Completeness [%] | 83.7 | 67.7 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.8 | 277 | 12% PEG8000 100MM IMIDAZOLE PH 6.8 150MM CACL2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MgADP | 2 (mM) | |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | reservoir | PEG8000 | 12 (%) | |
5 | 1 | reservoir | 150 (mM) | ||
6 | 1 | reservoir | imidazole | 100 (mM) | |
7 | 1 | drop | 8 (mM) |