1BQH
MURINE CD8AA ECTODOMAIN FRAGMENT IN COMPLEX WITH H-2KB/VSV8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1997-04-15 |
Detector | BRANDEIS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.900, 98.400, 170.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vaa |
RMSD bond length | 0.010 |
RMSD bond angle | 27.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.061 * | 0.249 * |
Total number of observations | 99259 * | |
Number of reflections | 34131 | |
<I/σ(I)> | 9.7 | 1.9 |
Completeness [%] | 93.8 | 90.7 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | 20-25% PEG 4K, 0.2M LI2SO4, 0.1M TRIS PH8.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | reservoir | PEG4000 | 20-25 (%) | |
4 | 1 | reservoir | 0.2 (M) | ||
5 | 1 | reservoir | Tris | 0.1 (M) |