1B90
BACILLUS CEREUS BETA-AMYLASE APO FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 291 |
Detector technology | AREA DETECTOR |
Collection date | 1997-01 |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.075, 92.103, 65.671 |
Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
Rwork | 0.164 |
R-free | 0.24700 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 24.250 * |
Data reduction software | SAINT |
Data scaling software | SAINT |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.578 |
High resolution limit [Å] | 2.500 | 2.449 |
Rmerge | 0.073 * | |
Total number of observations | 59931 * | |
Number of reflections | 24292 | |
Completeness [%] | 98.8 | 90 |
Redundancy | 2.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.6 | 18 * | drop consists of equal volume of protein and precipitant solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | acetate | 0.1 (M) | |
3 | 1 | reservoir | PEG6000 | 18 (%) | precipitant |
4 | 1 | reservoir | ammonium sulfate | 5 (%sat) | precipitant |
5 | 1 | reservoir | acetate | 0.1 (M) | precipitant |