1B7O

VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-08-04
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 21 21 21
Unit cell lengths	56.470, 61.470, 32.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.174 *
Rwork	0.174
Structure solution method	OTHER
Starting model (for MR)	WILD-TYPE HUMAN LYSOZYME
RMSD bond length	0.010
RMSD bond angle	1.570
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.076	0.190
Total number of observations	29226 *
Number of reflections	9887 *
<I/σ(I)>		3.1
Completeness [%]	89.5	75.8
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	10 *	Takano, K., (1995) J.Mol.Biol., 254, 62. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		2.5 (M)
3	1	reservoir	acetate	20 (mM)