1B47
STRUCTURE OF THE N-TERMINAL DOMAIN OF CBL IN COMPLEX WITH ITS BINDING SITE IN ZAP-70
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-12 |
| Detector | ADSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.960, 105.480, 84.920 |
| Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 24.530 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | HEAVY |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 * | |
| Total number of observations | 178845 * | |
| Number of reflections | 70918 | |
| <I/σ(I)> | 3.9 | 1.1 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 22 * | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 20 (%) | |
| 2 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
