1B2P
NATIVE MANNOSE-SPECIFIC BULB LECTIN FROM SCILLA CAMPANULATA (BLUEBELL) AT 1.7 ANGSTROMS RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02-13 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.420, 92.950, 46.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.186 * |
Rwork | 0.186 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gna |
RMSD bond length | 0.008 |
RMSD bond angle | 29.278 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((ROTAVATA)) |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.039 | |
Total number of observations | 161054 * | |
Number of reflections | 33837 | |
Completeness [%] | 98.5 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | HANGING-DROP VAPOUR-DIFFUSION METHOD WELL: 70% SATURATED AMMONIUM SULPHATE, PH 4.7 DROP: 5.5 MG/ML PROTEIN, 10MM DAP, 600MM PHOSPHATE BUFFERED SALINE, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 5.5 MG/ML PROTEIN | ||
2 | 1 | 1 | 10MM DAP | ||
3 | 1 | 1 | 600MM PHOSPHATE BUFFERED SALINE | ||
4 | 1 | 2 | 70% SATURATED AMMONIUM SULPHATE, PH 4.7 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | phosphate | 600 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 70 (%sat) |