1AS2
GDP+PI BOUND G42V GIA1
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 278 |
Detector technology | IMAGE PLATE |
Collection date | 1996-04 |
Detector | RIGAKU |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.190, 77.190, 143.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.25000 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1git |
RMSD bond length | 0.012 |
RMSD bond angle | 23.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.930 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.059 | 0.237 |
Number of reflections | 10403 | |
<I/σ(I)> | 15.4 | |
Completeness [%] | 93.1 | 95.2 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 20 * | THE PROTEIN WAS CRYSTALLIZED IN HANGING DROPS USING 3M (NH4)H2P04 AS THE PRECIPITANT (PH 5.6). 50 MM HEPES, PH 8.0, 10 MM MGSO4, 10 MM DTT AND 5 MM GDP WAS THE BUFFER, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-7.5 (mg/ml) | |
2 | 1 | drop | ammonium phosphate | 1.1-1.25 (M) | |
3 | 1 | reservoir | ammonium phosphate | 2.2-2.5 (M) |