1AQT
EPSILON SUBUNIT OF F1F0-ATP SYNTHASE FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 94.610, 94.610, 56.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 - 2.300 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.28800 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.059 * | 0.475 * |
| Total number of observations | 19833 * | |
| Number of reflections | 6626 | |
| <I/σ(I)> | 12.4 | 1.8 |
| Completeness [%] | 94.0 | 89 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | PROTEIN (4 MG/ML)IN 50 MM HEPES BUFFER, PH 7.5, 200 MM (NH2)2SO4, 2 M (NA/K)PO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2 (M) | ||
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | drop | protein | 2 (mg/ml) | |
| 5 | 1 | drop | 1 (M) | ||
| 6 | 1 | drop | HEPES | 50 (mM) | |
| 7 | 1 | drop | 100 (mM) |
