1ANG

CRYSTAL STRUCTURE OF HUMAN ANGIOGENIN REVEALS THE STRUCTURAL BASIS FOR ITS FUNCTIONAL DIVERGENCE FROM RIBONUCLEASE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	83.400, 120.600, 37.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.22
Rwork	0.220
RMSD bond length	0.015
RMSD bond angle	3.510
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.110 *
Total number of observations	41725 *
Number of reflections	7229 *
Completeness [%]	88.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium citrate	0.01 (M)
3	1	reservoir	sodium potassium tartrate	0.2 (M)
4	1	reservoir	PEG6000	20-25 (%)
5	1	reservoir	beta-octyl glucoside	0.5 (%)
6	1	reservoir	sodium citrate	0.01 (M)