1AMO
THREE-DIMENSIONAL STRUCTURE OF NADPH-CYTOCHROME P450 REDUCTASE: PROTOTYPE FOR FMN-AND FAD-CONTAINING ENZYMES
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1995-04-17 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.280, 116.180, 119.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.600 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.31000 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 24.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.760 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 * | 0.308 * |
Total number of observations | 171499 * | |
Number of reflections | 41132 | |
<I/σ(I)> | 13.5 | 2.4 |
Completeness [%] | 91.5 | 85.8 |
Redundancy | 4.2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 * | 19 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | reservoir | PEG4500 | 21 (%) | |
3 | 1 | reservoir | HEPES | 150 (mM) | |
4 | 1 | reservoir | 5 (mM) | ||
5 | 1 | reservoir | 0.8 (M) |