1AKH
MAT A1/ALPHA2/DNA TERNARY COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 94 |
Detector technology | IMAGE PLATE |
Collection date | 1995-05 |
Detector | RIGAKU |
Spacegroup name | P 61 |
Unit cell lengths | 132.250, 132.250, 45.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.500 |
R-factor | 0.201 * |
Rwork | 0.201 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yrn |
RMSD bond length | 0.014 |
RMSD bond angle | 1.870 |
Data reduction software | R-AXIS |
Data scaling software | R-AXIS |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.480 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.066 * | |
Total number of observations | 31013 * | |
Number of reflections | 15656 | |
Completeness [%] | 83.6 | 67 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 295 | 100MM HEPES PH 7.0, 20MM CACL2, 5MM [CO(NH3)6]CL3., VAPOR DIFFUSION, HANGING DROP, temperature 295.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | HEPES BUFFER | ||
3 | 1 | 1 | CACL2 | ||
4 | 1 | 1 | COBALT HEXAMINE | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | HEPES BUFFER | ||
7 | 1 | 2 | CACL2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 50 (mM) | |
2 | 1 | drop | 10 (mM) | ||
3 | 1 | drop | cobaltic hexamine | 2.5-5 (mM) | |
4 | 1 | reservoir | HEPES | 150-200 (mM) | |
5 | 1 | reservoir | 20 (mM) |