1AKD
CYTOCHROME P450CAM FROM PSEUDOMONAS PUTIDA, COMPLEXED WITH 1S-CAMPHOR
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1996-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.300, 66.200, 106.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.000 - 1.800 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED COORDINATES FROM TETRAGONAL FORM OF P450CAM |
| RMSD bond length | 0.009 |
| RMSD bond angle | 34.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.046 | 0.284 |
| Total number of observations | 143928 * | |
| Number of reflections | 41095 | |
| Completeness [%] | 92.0 | 69.4 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.4 | 4 * | PROTEIN WAS CRYSTALLIZED BY DIALYSIS FROM 10% PEG 8000, 50 MM TRISHCL PH 7.4, 250 MM KCL, 100 MM DTE, IN THE PRESENCE OF EXCESS (1S)-CAMPHOR., dialysis |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | DTE | 100 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 10 (%) |
