1AH6

STRUCTURE OF THE TETRAGONAL FORM OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-05
Detector	MARRESEARCH
Spacegroup name	P 43 2 2
Unit cell lengths	72.260, 72.260, 107.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.206
Rwork	0.206
R-free	0.25500
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	26.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.043
Number of reflections	26124
<I/σ(I)>	10.8	6.6
Completeness [%]	97.0	92.6
Redundancy	2.7	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4 *		Prodromou, C., (1996) Proteins: Struct.,Funct., Genet., 25, 517. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	36 (mg/ml)
2	1	1	PEGME550	3 (%)
3	1	1		20 (mM)
4	1	1	Tris	10 (mM)