1AGS
A SURFACE MUTANT (G82R) OF A HUMAN ALPHA-GLUTATHIONE S-TRANSFERASE SHOWS DECREASED THERMAL STABILITY AND A NEW MODE OF MOLECULAR ASSOCIATION IN THE CRYSTAL
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | SIEMENS-NICOLET X100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.500, 92.900, 115.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.31 |
Rwork | 0.310 |
Data reduction software | XENGEN (V. 2.1) |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.100 |
Number of reflections | 18098 |
Completeness [%] | 92.6 * |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 * | 18 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | |
3 | 1 | reservoir | PEG3350 | 18 (%) | |
4 | 1 | reservoir | EDTA | 1 (mM) | |
5 | 1 | reservoir | 1,2,3-heptanetriol | 0.05 (%(w/v)) | |
6 | 1 | reservoir | Tris-HCl | 100 (mM) |