1ACC
ANTHRAX PROTECTIVE ANTIGEN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1995-03-08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.300, 93.700, 82.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.100 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.29600 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.067 * | |
| Number of reflections | 45183 | |
| <I/σ(I)> | 9.6 | 4 |
| Completeness [%] | 91.1 | 84.3 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 4 * | PROTEIN WAS CRYSTALLIZED FROM 12-18% PEG 8K AND 50 MM CITRATE, PH 6.0 AT 4 DEGREES C. CRYSTAL FORM ""2"" (NEEDLE-LIKE CRYSTALS)., temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG18000 | 12-18 (%) | |
| 2 | 1 | 1 | citrate | 50 (mM) |
