1AB6
STRUCTURE OF CHEY MUTANT F14N, V86T
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-07 |
Detector | MARRESEARCH |
Spacegroup name | P 31 |
Unit cell lengths | 54.070, 54.070, 91.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.28000 |
Structure solution method | MOLECULAR |
Starting model (for MR) | 3chy |
RMSD bond length | 0.008 |
RMSD bond angle | 23.400 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.048 | 0.290 |
Total number of observations | 25441 * | |
Number of reflections | 13544 | |
Completeness [%] | 86.0 | 55.6 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 | 293 | THE PROTEIN WAS CRYSTALLIZED WITH 3.0M AMMONIUM SULFATE, PH7.2 AT 20C., temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.6-3.0 (M) | |
2 | 1 | drop | protein | 0.7 (mg/ml) | |
3 | 1 | drop | ammonium sulfate | 1.7-2.0 (M) | |
4 | 1 | reservoir | Tris-HCl | ||
5 | 1 | drop | Tris-HCl |