1A9W
HUMAN EMBRYONIC GOWER II CARBONMONOXY HEMOGLOBIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.800, 62.800, 320.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.186 * |
| Rwork | 0.185 |
| R-free | 0.23500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bbb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 19.500 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.091 | 0.417 * |
| Total number of observations | 118919 * | |
| Number of reflections | 14597 * | |
| <I/σ(I)> | 7.1 | 1.9 |
| Completeness [%] | 95.9 | 72.6 * |
| Redundancy | 5.9 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | HEPES | 0.01 (M) | |
| 3 | 1 | drop | dithionite | 2 (mM) | |
| 4 | 1 | reservoir | TAPS-KOH | 0.2 (M) | |
| 5 | 1 | reservoir | MME PEG5000 | 21 (%(w/v)) |
