1A7L
DOMINANT B-CELL EPITOPE FROM THE PRES2 REGION OF HEPATITIS B VIRUS IN THE FORM OF AN INSERTED PEPTIDE SEGMENT IN MALTODEXTRIN-BINDING PROTEIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.600, 71.300, 123.200 |
| Unit cell angles | 90.00, 94.90, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MBP RESIDUES 4 - 362 (PDB ENTRY 1ANF) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.800 * |
| Data reduction software | XDS |
| Data scaling software | MARSCALE |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.119 | 0.419 |
| Number of reflections | 24492 | 2146 * |
| <I/σ(I)> | 14.3 | 2.7 |
| Completeness [%] | 98.0 | 90 |
| Redundancy | 3.6 | 3.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | Vulliez-Le Normand, B., (1997) Protein Eng., 10, 175. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.0 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 26 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 4 | 1 | reservoir | maltose | 1 (mM) | |
| 5 | 1 | reservoir | 0.2 (M) |
