1A2D

PYRIDOXAMINE MODIFIED MURINE ADIPOCYTE LIPID BINDING PROTEIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-06
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	80.080, 96.250, 49.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.600 *
R-factor	0.196
Rwork	0.196
R-free	0.28500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lib
RMSD bond length	0.007 *
RMSD bond angle	1.395 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.520
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.095 *	0.302 *
Total number of observations	76938 *
Number of reflections	8916 *
<I/σ(I)>	13.1	2.3
Completeness [%]	94.0 *	78.05 *
Redundancy	4.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	reservoir	PEG400	20-30 (%)
4	1	reservoir		0.1-0.25 (M)
5	1	reservoir	Tris-HCl	0.1 (M)