1A0A
PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 288 |
Detector technology | DIFFRACTOMETER |
Collection date | 1996-06 |
Detector | WEISSENBERG |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.510, 68.300, 108.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.23 * |
Rwork | 0.230 |
R-free | 0.28400 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 23.900 * |
Data reduction software | WEIS |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.950 | |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.087 | 0.202 |
Total number of observations | 36943 * | |
Number of reflections | 9398 | |
<I/σ(I)> | 4.2 | 3.5 |
Completeness [%] | 92.0 | 76.8 * |
Redundancy | 3.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 3.6 | VAPOR DIFFUSION METHOD: DROP-0.4MM PROTEIN, 0.2MM DNA, 1% PEG6K, 20MM NACITRATE (PH3.6), RESERVOIR-1% PEG6K, 20MM NACITRATE(PH3.6), vapor diffusion |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
2 | 1 | 1 | NA CITRATE | ||
3 | 1 | 2 | PEG 6000 | ||
4 | 1 | 2 | NA CITRATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.4 (mM) | |
2 | 1 | drop | DNA | 0.2 (mM) | |
3 | 1 | drop | PEG6000 | 1 (%(w/v)) | |
4 | 1 | drop | sodium citrate | 20 (mM) | |
5 | 1 | reservoir | PEG6000 | 1 (%(w/v)) | |
6 | 1 | reservoir | sodium citrate | 20 (mM) |