10GS
HUMAN GLUTATHIONE S-TRANSFERASE P1-1, COMPLEX WITH TER117
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-08 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.723, 90.460, 69.404 |
| Unit cell angles | 90.00, 98.19, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.1762 |
| Rwork | 0.176 |
| R-free | 0.21460 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gss |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.079 * | 0.178 * |
| Total number of observations | 61110 * | |
| Number of reflections | 22104 * | |
| <I/σ(I)> | 12.5 | 17.8 |
| Completeness [%] | 90.0 * | 81 * |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 22 * | pH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | phosphate | 10 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | mercaptoethanol | 2 (mM) | |
| 5 | 1 | drop | TER-117 | ||
| 6 | 1 | reservoir | ammonium sulfate | 20-34 (%(w/v)) | |
| 7 | 1 | reservoir | dithiothreitol | 50-80 (mM) | |
| 8 | 1 | reservoir | MES | 100 (mM) |
