+Open data
-Basic information
Entry | Database: PDB / ID: 7eqi | ||||||
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Title | ChlB3 [Aceyltransferase] | ||||||
Components | ChlB3 | ||||||
Keywords | TRANSFERASE / Acyltransferase / ChlB3 | ||||||
Function / homology | 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III / 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III / 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminal / 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal / 3-oxoacyl-[acyl-carrier-protein] synthase activity / Thiolase-like / fatty acid biosynthetic process / ChlB3 Function and homology information | ||||||
Biological species | Streptomyces antibioticus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / FREE ELECTRON LASER / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.1 Å | ||||||
Authors | Saeed, A.U. / Zheng, J. | ||||||
Citation | Journal: Molecules / Year: 2022 Title: Structural Insight of KSIII ( beta-Ketoacyl-ACP Synthase)-like Acyltransferase ChlB3 in the Biosynthesis of Chlorothricin. Authors: Saeed, A.U. / Rahman, M.U. / Chen, H.F. / Zheng, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7eqi.cif.gz | 471.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7eqi.ent.gz | 385.4 KB | Display | PDB format |
PDBx/mmJSON format | 7eqi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eq/7eqi ftp://data.pdbj.org/pub/pdb/validation_reports/eq/7eqi | HTTPS FTP |
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-Related structure data
Related structure data | 4xsaS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 37017.992 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces antibioticus (bacteria) Production host: Streptomyces antibioticus JCM 4620 (bacteria) References: UniProt: Q0R4P5 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.63 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 6.5 / Details: 0.1 M HEPS pH 7.0, 1.4 M sodium acetate-trihydrate |
-Data collection
Diffraction | Mean temperature: 293.15 K / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: FREE ELECTRON LASER / Site: SACLA / Beamline: BL2 / Wavelength: 0.939 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS MYTHEN2 R 1K / Detector: PIXEL / Date: Oct 12, 2018 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.939 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 3.1→50 Å / Num. obs: 74294 / % possible obs: 99.5 % / Redundancy: 6 % / Rmerge(I) obs: 0.115 / Rpim(I) all: 0.051 / Rrim(I) all: 0.126 / Χ2: 0.936 / Net I/σ(I): 7.2 / Num. measured all: 442810 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4xsa Resolution: 3.1→50 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso max: 166.21 Å2 / Biso mean: 66.5365 Å2 / Biso min: 33.56 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→50 Å
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LS refinement shell | Resolution: 3.1→3.181 Å
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