EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural specializations of the sperm tail.
ジャーナル・号・ページ	Cell, Vol. 186, Issue 13, Page 2880-2896.e17, Year 2023
掲載日	2023年6月13日
著者	Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / Tzviya Zeev-Ben-Mordehai /
PubMed 要旨	Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule- ...Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule-based molecular machine-the axoneme-but it is unclear how axonemal microtubules are ornamented to support motility in diverse fertilization environments. Here, we present high-resolution structures of native axonemal doublet microtubules (DMTs) from sea urchin and bovine sperm, representing external and internal fertilizers. We identify >60 proteins decorating sperm DMTs; at least 15 are sperm associated and 16 are linked to infertility. By comparing DMTs across species and cell types, we define core microtubule inner proteins (MIPs) and analyze evolution of the tektin bundle. We identify conserved axonemal microtubule-associated proteins (MAPs) with unique tubulin-binding modes. Additionally, we identify a testis-specific serine/threonine kinase that links DMTs to outer dense fibers in mammalian sperm. Our study provides structural foundations for understanding sperm evolution, motility, and dysfunction at a molecular level.
リンク	Cell / PubMed:37327785 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 4.1 Å
構造データ	17187 8otz EMDB-17187, PDB-8otz: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm 手法: EM (単粒子) / 解像度: 3.6 Å 17188 8ou0 EMDB-17188, PDB-8ou0: bovine sperm endpiece singlet microtubules (one tubulin dimer and associated microtubule inner proteins) 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18071: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen top 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-18072: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen middle 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18073: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen bottom 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-18074: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 top 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18075: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 middle 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-18076: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 bottom 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18077: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 top 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-18078: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 middle 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-18079: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 bottom 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-18080: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 top 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-18081: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 middle 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-18082: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 bottom 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-18083: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 top 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-18084: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 middle 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-18085: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 bottom 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-18086: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction top 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-18087: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction middle 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18088: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction bottom 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-18089: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon top 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18090: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon middle 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-18091: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon bottom 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-18092: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction top 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-18093: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction middle 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-18094: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction bottom 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-18095: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 top 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-18096: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 middle 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-18097: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 bottom 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-18098: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 top 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-18099: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 middle 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-18100: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 bottom 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-18101: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 top 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-18102: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 middle 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-18103: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 bottom 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-18104: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 top 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-18105: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 middle 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-18106: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 bottom 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-18107: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, consensus refinement 手法: EM (単粒子) / 解像度: 4.0 Å 40619 8snb EMDB-40619: cryo-EM structure of sea urchin sperm doublet microtubule (48-nm periodicity) PDB-8snb: atomic model of sea urchin sperm doublet microtubule (48-nm periodicity) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-40620: cryo-EM density map of the 48-nm repeat doublet microtubule with beak from Chlamydomonas 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-40621: cryo-EM density map of the 48-nm repeat doublet microtubule without beak from Chlamydomonas 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸
由来	bos taurus (ウシ) cattle (ウシ) strongylocentrotus purpuratus (ムラサキウニ) purple sea urchin (ムラサキウニ) Chlamydomonas reinhardtii (クラミドモナス)
キーワード	STRUCTURAL PROTEIN (タンパク質) / microtubule (微小管) / cilia (繊毛) / sperm / axoneme / CYTOSOLIC PROTEIN (細胞質基質) / microtubule inner protein / doublet microtubule / DMT / flagella (鞭毛) / FAP / CFAP / MIP / MAP / cytoskeleton (細胞骨格) / tublin / sea urchin (ウニ) / TEX / Tektin / SAXO / ODF / SPATA / RIB