9Z2D

Mitochondrial Creatine Kinase in complex with ADP, creatine, and uncompetitive inhibitor uci

これはPDB形式変換不可エントリーです。

9Z2D の概要

• エントリーDOI: 10.2210/pdb9z2d/pdb
• EMDBエントリー: 73766
• 分子名称: Creatine kinase U-type, mitochondrial, ADENOSINE-5'-DIPHOSPHATE, N-[(E)-AMINO(IMINO)METHYL]-N-METHYLGLYCINE, ... (5 entities in total)
• 機能のキーワード: kinase, inhibitor, transferase-inhibitor complex, transition state analog, transferase/inhibitor
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 388478.63

構造登録者

Demir, M.,Zhao, J.,Sergienko, E. (登録日: 2025-11-04, 公開日: 2026-01-28)

主引用文献

Demir, M.,Ma, C.T.,Puvvula, N.,Koepping, L.,Gosalia, P.,Pollari, S.,Alba, S.,Zong, Z.,Li, Y.,Fujimoto, L.,Bobkov, A.,Hitosugi, T.,Zhao, J.,Sergienko, E.
Uncompetitive Allosteric Inhibitor of Mitochondrial Creatine Kinase Prevents Binding and Release of Creatine by Stabilization of Loop Closure.
Biorxiv, 2026

PubMed Abstract: Mitochondrial creatine kinase (MtCK) is a key enzyme in energy buffering and homeostasis in cells. It catalyzes transfer of phosphoryl group from ATP to creatine. Overexpression of MtCK occurs in many cancer cells to meet elevated energy demands, which is associated with poor prognosis. This suggests that MtCK may be a promising target for cancer therapeutics. We sought to discover first-in-class selective inhibitors of MtCK with diverse mechanisms of action using high-throughput screening, biochemical characterization and cryo-EM studies. Through these studies, we identified diverse types of compounds that modulate activity of MtCK , including fast-equilibrium and time-dependent orthosteric and allosteric inhibitors. Select hits were subjected to enzymatic and binding assays to assess MtCK inhibition and binding. A subset of inhibitors was advanced into structural studies using cryo-EM resulting in the molecular structure of MtCK with and without bound substrates in complex with an allosteric uncompetitive inhibitor that we discovered. These studies identified compound's unique binding pocket on MtCK and established the molecular steps manifesting in the apparent uncompetitive mode of inhibition. We demonstrate that the compound stabilizes an active site loop in a closed conformation restricting access of the creatine substrate to and the release of phosphocreatine product from its binding pocket. These findings establish chemical tools suitable for validation of MtCK as a promising breast cancer target with high therapeutic potential and build a foundation for future structure-guided optimization of the hit compounds of MtCK we identified and de novo rational design of novel MtCK inhibitors.

PubMed: 41542558
DOI: 10.64898/2026.01.08.696683

実験手法

ELECTRON MICROSCOPY (2.6 Å)