9YOL

Tra1 module of ctSAGA

9YOL の概要

• エントリーDOI: 10.2210/pdb9yol/pdb
• EMDBエントリー: 73261
• 分子名称: SCA7 domain-containing protein, Spt20-like SEP domain-containing protein, Chains: C, ... (6 entities in total)
• 機能のキーワード: ctsaga complex, histone acetyl transferase (hat) module, tra1 module and core module, transcription
• 由来する生物種: Thermochaetoides thermophila; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 893968.16

構造登録者

Mattoo, R.U.H.,Chen, D.H.,Bushnell, D.A.,Tamir, S.,Kornberg, R.D. (登録日: 2025-10-13, 公開日: 2025-12-03, 最終更新日: 2025-12-17)

主引用文献

Mattoo, R.U.H.,Chen, D.H.,Bushnell, D.A.,Tamir, S.,Kornberg, R.D.
Structure of the transcriptional co-activator SAGA complex, including the histone acetyltransferase module.
Mol.Cell, 85:4333-4346.e4, 2025

PubMed Abstract: The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex, a 1.8 MDa multi-subunit assembly comprising 19 subunits, is required for RNA polymerase II transcription in eukaryotes. The complex consists of four modules: transcription-associated protein 1 (Tra1), core, deubiquitination (DUB), and histone acetyltransferase (HAT). Although the structures of the Tra1, core, and DUB modules have been determined, the overall architecture of the HAT module remained elusive due to its inherent flexibility. To address this, we conducted cryo-electron microscopy (cryo-EM) analyses on SAGA purified from the thermophilic fungus Chaetomium thermophilum, yielding structures of Tra1 and core modules at 2.6 Å and three of the four HAT subunits at 3.7 Å. The structure of the HAT module was informative about the aspects of histone acetylation and the interface of HAT-core modules, contradicting earlier AlphaFold predictions. Our structure-guided genetic and biochemical analyses confirmed the roles of Ada1 and Spt7 in anchoring the HAT module within the SAGA complex.

PubMed: 41260211
DOI: 10.1016/j.molcel.2025.10.025

実験手法

ELECTRON MICROSCOPY (2.6 Å)