9Y4H

Structure of guinea pig ribosome with P/E-tRNA and mRNA

これはPDB形式変換不可エントリーです。

9Y4H の概要

• エントリーDOI: 10.2210/pdb9y4h/pdb
• EMDBエントリー: 72483
• 分子名称: 40S ribosomal protein S14, 40S ribosomal protein S27, FAU ubiquitin like and ribosomal protein S30 fusion, ... (79 entities in total)
• 機能のキーワード: guinea pig ribosome, ribosome
• 由来する生物種: Cavia porcellus (domestic guinea pig); 詳細
• タンパク質・核酸の鎖数: 78
• 化学式量合計: 3379762.27

構造登録者

Gutierrez-Vargas, C.,De, S.,Maji, S.,Liu, Z.,Nieb, M.,Seluanov, A.,Gorbunova, V.,Frank, J. (登録日: 2025-09-03, 公開日: 2026-02-18)

主引用文献

Gutierrez-Vargas, C.,De, S.,Maji, S.,Liu, Z.,Ke, Z.,Niess, M.,Seluanov, A.,Gorbunova, V.,Frank, J.
Structures of naked mole-rat, tuco-tuco, and guinea pig ribosomes-is rRNA fragmentation linked to translational fidelity?
Nucleic Acids Res., 54:-, 2026

PubMed Abstract: Ribosomes are central to protein synthesis in all organisms. In mammals, the ribosome functional core is highly conserved. Remarkably, two rodent species, the naked mole-rat (NMR) and tuco-tuco, display fragmented 28S ribosomal RNA (rRNA), coupled with high translational fidelity and long lifespan. The unusual ribosomal architecture in the NMR and tuco-tuco has been speculated to be linked to high translational fidelity. Here, we show, by single-particle cryo-electron microscopy, that despite the fragmentation of their rRNA, NMR and tuco-tuco ribosomes retain their core functional architecture. Compared to ribosomes of the guinea pig, a phylogenetically related rodent without 28S rRNA fragmentation, ribosomes of NMR and tuco-tuco exhibit poorly resolved density for certain expansion segments. In contrast, the structure of the guinea pig ribosome shows high similarity to the human ribosome. Enhanced translational fidelity in the NMR and tuco-tuco may stem from subtle, allosteric effects in dynamics, linked to rRNA fragmentation.

PubMed: 41603730
DOI: 10.1093/nar/gkag006

実験手法

ELECTRON MICROSCOPY (4.7 Å)