9XPG

The structure of gp139 protein of phage phikz

9XPG の概要

• エントリーDOI: 10.2210/pdb9xpg/pdb
• EMDBエントリー: 67097
• 分子名称: PHIKZ139 (1 entity in total)
• 機能のキーワード: baseplate, inner peripheral, viral protein
• 由来する生物種: Pseudomonas phage phiKZ
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 130767.71

構造登録者

Xiao, H.,Peng, Z.,Zhou, J.,Liu, H. (登録日: 2025-11-16, 公開日: 2026-02-11, 最終更新日: 2026-06-24)

主引用文献

Xiao, H.,Peng, Z.,Zhou, J.,Chen, Y.,Peng, Y.,Tang, Y.,Li, T.,Chen, W.,Huang, S.Y.,Cheng, L.,Liu, H.
Structural atlas of the intact jumbo phage phiKZ.
Nat Commun, 17:-, 2026

PubMed Abstract: Jumbo phage phiKZ, a key model for studying phage nucleus formation and bacterial defense mechanisms, possesses a highly complex tail machine that is essential for infection. Here, we present the structural atlas of the intact jumbo phage phiKZ by cryo-EM, thereby identifying 40 constituent proteins and unveiling its modular architecture. The virion, with a length of approximately 360 nm, is comprised of an icosahedral capsid of 2520 polypeptide chains from 11 proteins, and a massive tail machine of over 900 polypeptide chains from 29 proteins. The tail features a unique, multi-layered neck and a highly elaborate baseplate. The neck is reinforced by whisker-like proteins and anchors the contractile tail, which terminates in the baseplate. The baseplate is constituted by a central hub, an inner periphery of interlocking wedge heterotrimers and hexagonal rings, and an outer periphery with a striking hexagonal star configuration. This intricate peripheral region of the baseplate serves as an extended platform for twelve peripheral fibers, which mediate host cell adsorption. Our findings provide a structural framework for understanding jumbo phage assembly and infection, thus contributing to the foundation for future functional studies and rational engineering of these phages for potential therapeutic applications.

PubMed: 41974715
DOI: 10.1038/s41467-026-71561-2

実験手法

ELECTRON MICROSCOPY (4.14 Å)