9X6W

Crystal structure of NodD-EBD (Effector Binding Domain) from Rhizobium leguminosarum bv. vicae 3841

9X6W の概要

• エントリーDOI: 10.2210/pdb9x6w/pdb
• 分子名称: LysR family transcriptional regulator (2 entities in total)
• 機能のキーワード: nodd, lysr transcription factors, flavonoids, rhizobia, transcription
• 由来する生物種: Rhizobium leguminosarum
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 201667.33

構造登録者

Ruan, Y.,Dong, S.,Zhang, Y.,Murray, J. (登録日: 2025-10-16, 公開日: 2025-12-24, 最終更新日: 2026-01-21)

主引用文献

Ruan, Y.,Dong, S.,Jiang, S.,Wang, Y.,Wu, X.,Zhuang, Y.,Wu, W.,East, A.K.,Xu, P.,Poole, P.S.,Zhang, Y.,Murray, J.D.
The molecular basis of the binding and specific activation of rhizobial NodD by flavonoids.
Science, 391:184-189, 2026

PubMed Abstract: The specific partnership between legumes and rhizobia relies on a chemical dialogue. Plant flavonoids activate the bacterial transcription factor NodD, which triggers production of Nod factors that are recognized by the plant. Structural studies of the (pea) symbiont NodD revealed two pockets that are essential for its activation by flavonoids. Comparative studies with NodD1 of , the symbiont of , revealed that this specificity is determined by the shape of the pocket and by specific amino acids. A chimeric NodD containing the flavonoid recognition residues from NodD1 in the NodD backbone was sufficient to complement nitrogen fixation in by an mutant, confirming the critical role of flavonoid recognition in host range.

PubMed: 41505558
DOI: 10.1126/science.aec3061

実験手法

X-RAY DIFFRACTION (3.3 Å)