9WNR

Structure of E.coli ribosome in complex with an engineered arrest peptide and trigger factor

これはPDB形式変換不可エントリーです。

9WNR の概要

• エントリーDOI: 10.2210/pdb9wnr/pdb
• EMDBエントリー: 66122
• 分子名称: Large ribosomal subunit protein bL28, 30S ribosomal protein S2, 30S ribosomal protein S3, ... (58 entities in total)
• 機能のキーワード: ribosomal nascent chain, ribosomal arrest peptide, ribosomal translation, ribosome stalling, 70s ribosome, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 57
• 化学式量合計: 2261020.03

構造登録者

Sriramoju, M.K.,Ko, T.P.,Draczkowski, P.,Hsu, S.T.D. (登録日: 2025-09-05, 公開日: 2025-10-22, 最終更新日: 2025-10-29)

主引用文献

Sriramoju, M.K.,Ko, T.P.,Draczkowski, P.,Hsu, S.D.
Structural basis of enhanced stalling efficiency of an engineered ribosome arrest peptide.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Ribosome stalling is a critical regulatory mechanism in protein synthesis, controlling the rate and fidelity of translation. Arrest peptides, short sequences within nascent chains, can induce ribosome stalling, providing insights into the dynamics of translation and potential therapeutic targets. In this study, we investigated the molecular mechanisms of ribosome stalling induced by an engineered ribosomal arrest peptide (eRAP). We used cryo-electron microscopy and biochemical assays to characterize the interactions between eRAP, the ribosome, and accessory factors such as the trigger factor. Our results reveal intricate details of the eRAP-induced ribosome stalling, including the conformational changes in the ribosomal tunnel and the nascent chain. We also observed interactions between eRAP and specific ribosomal components, highlighting the role of key amino acids in mediating ribosome stalling. Furthermore, comparison with other stalling mechanisms, such as those induced by antibiotics or natural nascent peptides, elucidates the unique features of eRAP-induced stalling. Overall, our findings provide a comprehensive understanding of ribosome stalling by arrest peptides, shedding light on the fundamental processes of translation and offering potential avenues for therapeutic interventions targeting translation regulation.

PubMed: 41099701
DOI: 10.1093/nar/gkaf978

実験手法

ELECTRON MICROSCOPY (2.76 Å)