9W2Y

Cryo-EM structure of complex III on the bovine heart submitochondrial particles, III-2

9W2Y の概要

• エントリーDOI: 10.2210/pdb9w2y/pdb
• EMDBエントリー: 65584
• 分子名称: Cytochrome b-c1 complex subunit 1, mitochondrial, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit 10, ... (14 entities in total)
• 機能のキーワード: respiratory chain complex, supercomplex, submitochondrial particles, motor protein, oxidoreductase
• 由来する生物種: Bos taurus (domestic cattle); 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 472886.09

構造登録者

Nakano, A.,Masuya, T.,Akisada, S.,Ishikawa-Fukuda, M.,Mitsuoka, K.,Miyoshi, H.,Murai, M.,Yokoyama, K. (登録日: 2025-07-28, 公開日: 2026-04-01)

主引用文献

Nakano, A.,Masuya, T.,Akisada, S.,Ishikawa-Fukuda, M.,Mitsuoka, K.,Miyoshi, H.,Murai, M.,Yokoyama, K.
Structures of respiratory supercomplexes and ATP synthase oligomers in mammalian mitochondrial inner membrane.
Nat Commun, 2026

PubMed Abstract: Understanding the functional mechanisms of membrane protein complexes requires structural analysis within their native membrane environment. Here, we applied cryo-electron microscopy to determine the structures of FF ATP synthase and respiratory supercomplexes (SCs) on sub-mitochondrial particles (SMPs) isolated from bovine heart mitochondria. Most FF complexes were observed as dimers stabilized by the regulatory factor IF₁, and a tetrameric assembly comprising two FF-IF₁ dimers arranged linearly was also identified. This finding indicates that the tetrameric units of FF are present in the mitochondrial inner membrane and contribute to shaping cristae tips in mammalian mitochondria. F domain maps resolve the e-subunit- c₈-ring interface and show no discrete density for a tightly bound lipid within the c₈-ring. In addition to the previously reported SCs compositions CI₁CIII₂CIV₁ and CI₁CIII₂CIV₂, our analysis identified an additional assembly with the composition CI₁CIII₂CIV₃, as well as a CI₂CIII₂CIV₆ mega-complex. This approach enables rapid structural determination of FF ATP synthase and SCs from minimal membrane fractions, providing a foundation for elucidating the molecular basis of metabolic disorders and mitochondrial diseases at the level of higher-order architecture.

PubMed: 41844608
DOI: 10.1038/s41467-026-70578-x

実験手法

ELECTRON MICROSCOPY (2.4 Å)