9W1C

LH2 complex from Ectothiorhodospira haloalkaliphila with inhibited carotenoid biosynthesis

これはPDB形式変換不可エントリーです。

9W1C の概要

• エントリーDOI: 10.2210/pdb9w1c/pdb
• EMDBエントリー: 65526
• 分子名称: Light-harvesting protein B:800-850 subunit beta, Light-harvesting protein B-800/850 alpha chain, BACTERIOCHLOROPHYLL A, ... (5 entities in total)
• 機能のキーワード: photosynthesis, light-harvesing, purple sulfur bacteria, cryo-em
• 由来する生物種: Ectothiorhodospira haloalkaliphila ATCC 51935; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 132828.70

構造登録者

Burtseva, A.D.,Baymukhametov, T.N.,Popov, V.O.,Ashikhmin, A.A.,Boyko, K.M. (登録日: 2025-07-25, 公開日: 2025-10-22, 最終更新日: 2026-02-04)

主引用文献

Burtseva, A.D.,Baymukhametov, T.N.,Bolshakov, M.A.,Starodubov, A.S.,Zhang, H.,Popov, V.O.,Ashikhmin, A.A.,Boyko, K.M.
Structural insights into LH2 complexes formed by a purple sulfur bacterium with inhibited carotenoid biosynthesis.
Febs Lett., 600:188-200, 2026

PubMed Abstract: The LH2 complex is essential for light harvesting in many photosynthetic bacteria. To elucidate the specific structural role of carotenoids, we analyzed LH2 complexes from Ectothiorhodospira haloalkaliphila with inhibited carotenoid biosynthesis. This approach allowed us to study complexes incorporating the colorless carotenoid phytoene instead of the native, colored pigments. A 1.92 Å cryo-EM reconstruction revealed that phytoene fully substitutes for the native carotenoids while maintaining the octameric symmetry of the complex and the precise arrangement of bacteriochlorophylls. These results demonstrate that the architectural function of carotenoids in LH2 complexes is maintained even when their light-absorption capability is altered, providing new mechanistic insight into the structural basis of pigment-protein interactions in photosynthetic antenna complexes.

PubMed: 41313718
DOI: 10.1002/1873-3468.70242

実験手法

ELECTRON MICROSCOPY (1.92 Å)