9VYF

Cyanopodophage Pan3 portal complex

9VYF の概要

• エントリーDOI: 10.2210/pdb9vyf/pdb
• EMDBエントリー: 65451
• 分子名称: Portal protein (1 entity in total)
• 機能のキーワード: cyanophage, virus, portal, viral protein
• 由来する生物種: Pseudanabaena phage Pan3
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 831854.44

構造登録者

Hou, P.,Zhou, C.Z.,Jiang, Y.L. (登録日: 2025-07-21, 公開日: 2025-11-26, 最終更新日: 2026-01-28)

主引用文献

Hou, P.,Zhu, J.,Yu, R.C.,Yang, F.,Du, K.,Li, J.,Kong, W.W.,Wang, J.,Chen, Y.,Zhou, C.Z.,Jiang, Y.L.
Cryo-EM structure of cyanopodophage Pan3 reveals a modular tail architecture for host recognition.
Structure, 2025

PubMed Abstract: Cyanophages, which are bacteriophages that specifically infect host cyanobacteria, also utilize the tail to initiate host recognition and adsorption. Owing to the limited structural information on cyanophages, our understanding of the mechanism by which cyanophages specifically recognize their hosts remains largely unknown. Here, we determined the intact cryoelectron microscopy structure of a freshwater cyanopodophage Pan3, which consists of an icosahedral shell and a short tail comprising four modular components: the dodecameric adaptor, hexameric nozzle, trimeric needle, and six heterohexameric tailspikes. Notably, each tailspike features an SGNH esterase domain fused to a lectin domain, forming a continuous groove complementary to the host lipopolysaccharide. These findings provide insights into the receptor engagement in Podoviridae, and establish a structural framework for cyanophage and host interactions that may guide future antibacterial interventions against harmful blooms.

PubMed: 41380677
DOI: 10.1016/j.str.2025.11.012

実験手法

ELECTRON MICROSCOPY (3.3 Å)