9VSD
Structure of the phosphomimetic mutant CAX1 in Arabidopsis thaliana in the apo state
Summary for 9VSD
| Entry DOI | 10.2210/pdb9vsd/pdb |
| EMDB information | 65301 |
| Descriptor | Vacuolar cation/proton exchanger 1 (1 entity in total) |
| Functional Keywords | calcium;transporter;proton;cax1, transport protein |
| Biological source | Arabidopsis thaliana (thale cress) |
| Total number of polymer chains | 3 |
| Total formula weight | 153964.23 |
| Authors | |
| Primary citation | Wang, K.,Ma, C.,Chen, G.,Yang, Z.,Gao, Y.,Zhang, Z.,Liu, X.,Sun, L. Structural basis of CAX1 autoinhibition by its amino-terminal domain in Arabidopsis thaliana. Nat.Plants, 11:2072-2083, 2025 Cited by PubMed Abstract: Calcium homeostasis is tightly regulated due to the essential roles of calcium ions (Ca) in various cellular processes. CAX1 in Arabidopsis thaliana (AtCAX1) serves as a Ca/H exchanger transporting excess cytosolic Ca into the vacuole, which is modulated by kinase phosphorylation in response to diverse signals. However, the regulatory mechanism remains unclear. Here we present the structures of wild-type AtCAX1 in an inactivated state and a phosphomimetic mutant in an activated state. In the wild-type structure, the amino-terminal region forms an α-helix that blocks the transport tunnel, thus inhibiting its transport activity. In contrast, in the phosphomimetic mutant structure, this blocking helix is released from the tunnel, leading to AtCAX1 activation. Conformational changes are also observed in the transmembrane domain. Together, these findings provide insights into the transport mechanism of the Ca/H exchangers and set up a basis for future studies of the regulation of calcium homeostasis in plants. PubMed: 40897811DOI: 10.1038/s41477-025-02104-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.4 Å) |
Structure validation
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