9VS3

Apo structure of Ebinur lake virus polymerase

9VS3 の概要

• エントリーDOI: 10.2210/pdb9vs3/pdb
• EMDBエントリー: 65295
• 分子名称: RNA-directed RNA polymerase L (1 entity in total)
• 機能のキーワード: viral protein, polymerase
• 由来する生物種: Ebinur lake virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 259756.97

構造登録者

Tang, J.,Deng, Z. (登録日: 2025-07-08, 公開日: 2026-05-13, 最終更新日: 2026-05-27)

主引用文献

Tang, J.,Kuang, W.,Chen, W.,Wu, H.,Wang, Y.,Li, J.,Yuan, Z.,Xia, H.,Zhao, H.,Deng, Z.
Structural basis for RNA synthesis and inhibition of the orthobunyavirus polymerase.
Nat Commun, 2026

PubMed Abstract: Orthobunyaviruses are segmented negative-sense RNA viruses that encompass several life-threatening human and animal pathogens. However, no licensed antivirals are currently available. The viral RNA-dependent RNA polymerase (RdRp) is a multi-domain enzyme critical for genome replication and transcription, representing a promising target for antiviral drug development. Here, we establish robust in vitro enzymatic activity assays for the Ebinur Lake virus (EBIV) polymerase and identify suramin, a century-old drug, as an inhibitor of EBIV polymerase. We further determine cryo-EM structures of the EBIV polymerase in apo, elongation, and suramin-bound states. These structures reveal conformational rearrangements of the polymerase during RNA synthesis, including conformational transitions of the prime-and-realign (PR) loop and a unique β-hairpin that bridges the zinc-binding domain (ZBD) to the RdRp core. The structural observations are correlated with in vitro enzymatic activity and cell-based minireplicon assays. The suramin-bound structure reveals two distinct inhibitory binding sites. One site sterically clashes with the vRNA promoter. The other site directly blocks RNA template strand binding, thereby inhibiting polymerase activity. These findings advance our understanding of orthobunyavirus RNA synthesis mechanisms and offer a structural framework for the rational design and optimization of antiviral drugs.

PubMed: 42140913
DOI: 10.1038/s41467-026-72944-1

実験手法

ELECTRON MICROSCOPY (3.55 Å)