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9VLH

Crystal structure of Bacillus subtilis DegQ tetramer

Summary for 9VLH
Entry DOI10.2210/pdb9vlh/pdb
Related9VLI
DescriptorDegradation enzyme regulation protein DegQ (2 entities in total)
Functional Keywordstranscription regulatory protein, transcription
Biological sourceBacillus subtilis
Total number of polymer chains8
Total formula weight44443.55
Authors
Fujimoto, Z.,Kishine, N.,Kimura, K. (deposition date: 2025-06-25, release date: 2025-09-24, Last modification date: 2025-10-08)
Primary citationFujimoto, Z.,Kishine, N.,Saitou, K.,Kimura, K.
Tetrameric structure of Bacillus subtilis DegQ and its predicted interaction with the DegS-DegU two-component system.
Acta Crystallogr.,Sect.F, 81:425-433, 2025
Cited by
PubMed Abstract: Bacillus subtilis DegQ is a 46-amino-acid regulatory protein involved in the DegS-DegU two-component system. DegQ promotes the phosphorylation of DegU by DegS, switching the function of DegU from competence to the induction of poly-γ-glutamate production. To elucidate its structural role, we determined the crystal structures of wild-type DegQ and its mutant DegQS25L. Each DegQ monomer folds into a single α-helix, and four monomers assemble into a tetramer characterized by a four-helix coiled-coil structure. Within the tetramer, two adjacent helices are oriented in the same direction, while the other two are oriented oppositely, forming a pseudo-twofold symmetric arrangement. The mutant form displays disrupted symmetry due to altered helix packing, which is caused by shifts in the coiled-coil heptad register induced by the mutation. Structural predictions using AlphaFold3 suggest that DegQ likely binds to the N-terminal helix bundle of DegS, either as a dimer or as individual monomers. These findings provide structural insight into DegQ oligomerization and its potential role in modulating DegS autophosphorylation and DegU binding.
PubMed: 40937771
DOI: 10.1107/S2053230X25007903
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.3 Å)
Structure validation

243083

數據於2025-10-15公開中

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