9V1K

Cryo- EM structure of small subunit (head) of 75S ribosome with P- tRNA from Entamoeba histolytica

これはPDB形式変換不可エントリーです。

9V1K の概要

• エントリーDOI: 10.2210/pdb9v1k/pdb
• EMDBエントリー: 64696
• 分子名称: 40S ribosomal protein S25, 40S ribosomal protein S16, putative, 40S ribosomal protein S17, putative, ... (14 entities in total)
• 機能のキーワード: ssu-head, ribosome, p-trna, entamoeba histolytica
• 由来する生物種: Entamoeba histolytica HM-1:IMSS; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 853362.07

構造登録者

Sharma, S.,Mishra, S.,Gourinath, S.,Kaushal, P.S. (登録日: 2025-05-19, 公開日: 2025-10-08)

主引用文献

Sharma, S.,Mishra, S.,Gourinath, S.,Kaushal, P.S.
Cryo-EM structure of ribosome from pathogenic protozoa Entamoeba histolytica reveals unique features of its architecture.
Nat Commun, 16:7758-7758, 2025

PubMed Abstract: Entamoeba histolytica, an anaerobic protozoan parasite, is the causative agent of amoebiasis, bloody diarrhea, and liver abscesses in humans. Amoebiasis is more predominant in tropical areas with poor sanitation conditions, and it remains the fourth leading cause of death due to a protozoan infection. E. histolytica life cycle spans between an infective 'cyst stage' and an active disease-causing 'trophozoite stage'. We have isolated ribosomes from the trophozoite stage of E. histolytica. Here, we report single particle cryo-EM structures of the 53S ribosome large subunit (LSU), and 75S associated ribosomes, with P-tRNA, A/P and P/E tRNAs, and with paromomycin antibiotic, at 2.8 Å to 3.4 Å resolution. The E. histolytica possesses a reduced ribosome with a conserved core, and the periphery evolved with species-specific unique features. The most notable features are the presence of the rRNA triple helix near the peptide exit tunnel, the expansion segment H88ES102 near the exit site on LSU, and unique insertions in r-proteins. Furthermore, the 75S ribosome paromomycin complex structure provides the atomic details of its interactions. These structures provide insights into the evolutionary adaptation of the E. histolytica translational machinery and may be explored further for amoebicidal therapeutic intervention.

PubMed: 40835601
DOI: 10.1038/s41467-025-62767-x

実験手法

ELECTRON MICROSCOPY (3.4 Å)