9V0U

GPR133-Gain-miniG13 complex

Summary for 9V0U

• Entry DOI: 10.2210/pdb9v0u/pdb
• EMDB information: 64672
• Descriptor: Adhesion G-protein coupled receptor D1, Guanine nucleotide-binding protein subunit alpha-13,Isoform 2 of Guanine nucleotide-binding protein subunit alpha-13, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, ... (4 entities in total)
• Functional Keywords: gpcr, g12/g13, complex, stachel, membrane protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 138039.87

Authors

Xi, Y.,Pu, X.,Ping, Y. (deposition date: 2025-05-19, release date: 2025-07-30)

Primary citation

Pu, X.,Xi, Y.T.,Wang, M.X.,Zhang, D.,Ping, Y.Q.,Xiao, P.,Sun, J.P.
Cryo-EM structural elucidation and molecular mechanism of the GPR133-G13 signaling complex.
Biochem.Biophys.Res.Commun., 777:152165-152165, 2025

PubMed Abstract: GPR133 is an adhesion-class G protein-coupled receptor (GPCR) that has recently been de-orphanized. Its functions are complex and multifaceted. While GPR133 is primarily recognized for coupling with the Gs subunit to mediate elevated intracellular cAMP levels, its potential engagement with alternative signaling pathways remains poorly characterized. In our experiments, we demonstrated that GPR133 exhibits constitutive self-activation via its Stachel sequence as an adhesion GPCR, enabling activation of downstream G13 signaling. We reconstituted the GPR133-GAIN-miniGα13 complex in vitro and resolved its cryo-electron microscopy structure at a resolution of 3.51 Å. Detailed structural comparisons between the GPR133-GAIN-miniGα13 complex and the previously resolved GPR133-CTF-Gs structure highlighted both conserved and different features. These findings provide critical insights into the signal transduction mechanisms of GPR133 and lay a foundation for targeted therapeutic strategies.

PubMed: 40570642
DOI: 10.1016/j.bbrc.2025.152165

Experimental method

ELECTRON MICROSCOPY (3.51 Å)